![]() This demonstrated inheritance and resolved one case of an ambiguous paternity. The new genotypes, which increased the allelic diversity and heterozygosity in Gombe by 61% and 8%, respectively, were validated by replicate amplification and pedigree analyses. Applying this method to the entire Gombe population, we confirmed previously reported genotypes, but also identified 31 new alleles that had been missed due to sequence differences and size homoplasy. Using data from eight microsatellite loci as a reference, we designed a bioinformatics platform that converts raw MiSeq reads into locus‐specific files and automatically calls alleles after filtering stutter sequences and other PCR artifacts. ![]() Here we developed a MiSeq‐based approach and tested its performance using previously genotyped fecal samples from long‐term studied chimpanzees in Gombe National Park, Tanzania. ![]() However, this technique is labor intensive, difficult to compare across platforms, and notoriously imprecise. Until recently, capillary electrophoresis has been the method of choice to determine the length of polymorphic STR loci. Press “OK” to reboot.After the VERUS boots press “OK” on the CommPort error pop up box.On the VERUS Server popup select “Disable launch at startup” check box.Short tandem repeats (STRs), also known as microsatellites, are commonly used to noninvasively genotype wild‐living endangered species, including African apes. This Process will take approximately 10-15 min. ![]() ![]() Continue to hold the Left “Alt” button down until the “Loading RAMDISK Image” screen is displayed.Press “OK” on the System Recovery popup box. All 3rd party software will be uninstalled and/or deleted.Do not perform this process unless you have been instructed to do so by a Snap-on Representative.This process requires access to a USB Keyboard and an Internet connection to the VERUS.The VERUS will not resume normal operation until it has been successfully connected to the internet and Snap-on Diagnostics Technical Support has been allowed to remotely access the unit.Power off the VERUS.Plug in a USB keyboard.Press and hold the LEFT “Alt” button down and then press the VERUS’ power button once. All saved user files and documents will be deleted. Overview:How to re-image the VERUS Resolution:WARNING: This procedure will restore the unit to its factory settings. ![]()
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December 2022
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